Microscopy and culturing microorganisms

Biology · Cell biology. The idea in plain words, the rules to remember, the mistake to avoid and a worked example, written and checked by Mega Lecture.

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The idea

A microscope makes an image that is bigger than the real object. Magnification is how many times bigger the image is. Resolution is the ability to see two close points as separate points. An electron microscope has a much higher magnification and resolution than a light microscope, so it shows small structures such as ribosomes in detail.

Bacteria multiply by binary fission: one cell splits into two. With enough nutrients and a suitable temperature this can happen every 20 minutes, so the number doubles at each division.

To grow one type of bacterium only, we use aseptic technique. It stops other microorganisms from getting into the culture.

Rules to remember

Common mistake

Students divide an image size in mm by a real size in µm without converting. Change both to the same unit before you divide, and halve the diameter before you use πr2.

Worked example

In a photograph taken with a microscope, a cell is 45 mm wide. The magnification is ×900. What is the real width of the cell in µm?

  1. Real size = image size ÷ magnification.
  2. 45 mm ÷ 900 = 0.05 mm.
  3. Change to micrometres: 0.05 × 1000 = 50 µm.

Answer: 50 µm.

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